The Paediatric Biobank for Research, as an institution in charge of the pre-analytical process and custody of donor samples, offers technical services as well as ethical and legal advice on these samples.
Molecular techniques
Seguint protocols normalitzats de treball, rigorosament elaborats, el BHISJD, ofereix els següents serveis tècnics:
- DNA extraction from peripheral blood
- Saliva DNA extraction
- DNA extraction from frozen tissue
- DNA extraction from paraffinized tissues
- RNA extraction from peripheral blood
- RNA extraction from frozen tissue
- Plasma insulation
- Serum isolation
- Aliquoted urine
- Aliquoted Buffy Coat
- LCR centrifugation and/or aliquotation
- Western Blot + antibody optimization
- Western Blot
Histological techniques
- Inclusion of tissue in OTC and freezing
- Inclusion of tissue in paraffin and block production
- Microsections of frozen tissue (first section)/slide holder *
- Microsections of frozen tissue (adicinals)/slide holder *
- Microsections of frozen tissue/tube *
- Microsections of paraffin tissue (first section) *
- Microsections of paraffin tissue (additional) *
- Microsections of paraffin tissue/tube *
- Hematoxylin-eosin staining
- Histochemical staining
- Automated immunohistochemical staining
- Manual immunohistochemical staining
- Immunofluorescence staining in tissues
- Immunofluorescence staining in cell cultures
- Double immunofluorescence staining
- Antibody optimization
Optical microscopy and fluorescence techniques
- Self-service optical microscopy image capture *
- Optical microscopy image capture with support *
- Self-service fluorescence microscopy imaging *
- Capture images with fluorescence microscopy with support
- Capture images confocal microscopy
Cell culture techniques
- Purification and freezing of PBMCs
- Cultivation of fibroblasts
- Cryopreservation of fibroblasts
Registration and custody service
Once the donor has been duly informed and the Biobank has signed informed consent, the donation is recorded in specific software. The data associated with each donation are subject to the Data Protection Act. All samples are stored in the corresponding devices and stored under the responsibility of the BHISJD.
Sample transfer service
Go to the Applications for samples section
Counselling service
The Paediatric Biobank for Research provides legal and ethical counselling services to researchers on sample handling, collection creation, sample requests to other biobanks, and more.